Antimicrobial Peptide AP02231

     
 

APD ID:

AP02231

 
 

Name/Class:

KDAMP 19-mer (KAMP-19; keratin-derived AMPs; Gly-rich; human; primates, mammals, animals. Several other shorter derivatives were isolated: KAMP-18N, KAMP-18C, KAMP-17, KAMP-14, and KAMP-13)

 
 

Source:

corneas, eyes, Homo sapiens

 
 

Sequence:

RAIGGGLSSVGGGSSTIKY

 
 

Length:

19

 
 

Net charge:

2

 
 

Hydrophobic residue%:

26%

 
 

Boman Index:

0.55 kcal/mol

 
 

3D Structure:

nonhelixbeta

 
 

Method:

NMR

 
 

SwissProt ID:

PDB ID: 5KI0   Go to PDB

 
 

Activity:

Anti-Gram-,

 
 

Crucial residues:

glycines

 
 

Additional info:

Significance: Mild Keratin 6A knockdown in mouse cornea led to a substantial increase of bacterial colonization on the otherwise normal ocular surface. Active against P. aeruginosa. Because the short active fragments contain part of the 19mer sequence, they are all collected in the same entry here to increase the uniqueness of the database peptides. The 13mer is as active against P. aeruginosa as the 19-mer, suggesting the Gly-rich domain is essential. The 3D structure of KAMP-19 has been determined in the presence of SDS micelles.You can rotate, zoom, and view the 3D structure here in the PDB.

The amino acid sequeces of these fragments are below:

KAMP-18N, the 18-mer-N (sequence: _AIGGGLSSVGGGSSTIKY);

KAMP-18C: the 18-mer-C (sequence: RAIGGGLSSVGGGSSTIK);

KAMP-17 (sequence: _AIGGGLSSVGGGSSTIK);

KAMP-14 (sequence: ___GGGLSSVGGGSSTIK);

KAMP-13 (sequence: _AIGGGLSSVGGGS).

Read the comments of this work here. Updated 6/2016; 6/2017.
 
       
 

Title:

Cytokeratins mediate epithelial innate defense through their antimicrobial properties

 
 

Author:

Tam C, Mun JJ, Evans DJ, Fleiszig SM.2012

 
 

Reference:

J Clin Invest. 2012 Oct 1;122(10):3665-77. Pub-Med.

 
       

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