Antimicrobial Peptide AP02204

     
 

APD ID:

AP02204

 
 

Name/Class:

Colistin (Polymyxin E1 and E2; lactam, XXD; XXL; XXJ; UCSB1a; lipopeptides; nonribosomally synthesized peptide antibiotic; bacteria; BBL; BBMm, prokaryotes)

 
 

Source:

Paenibacillus polymyxa var. colistinus; Also known as Bacillus polymyxa

 
 

Sequence:

KTKKKLLKKT

 
 

Length:

10

 
 

Net charge:

6

 
 

Hydrophobic residue%:

20%

 
 

Boman Index:

2.86 kcal/mol

 
 

3D Structure:

nonhelixbeta

 
 

Method:

NMR

 
 

SwissProt ID:

Reference ID: Ref  

 
 

Activity:

Anti-Gram-, Antibiofilm,

 
 

Crucial residues:

The fatty acid is critical for bacterial killing but not LPS binding.

 
 

Additional info:

Warning: the sequence is a close mimic of the natural peptide antibiotic. In the natural form, there are multiple Dab (2,4-diaminobutanoic acid), which are represented here as lysines. The molecule is cyclic due to the formation of an amide bond between the amino group of Dab in position 4 and the carboxyl group of the C-terminus. Residue Leu6 is a D-amino acid and the N-terminus is attached by a fatty acid (fatty acid A=6-methyloctanoic acid; B=6-methylheptanoic acid). It preferentially binds to LPS of Gram-negative bacteria and disrupts membranes (Moser et al., 2014). Because of such interesting properties, there is interest in developing polymyxin analogs. AMPs in use: Colistin, used alone or in combination, is important to treat Gram-negative infections such as CF involving P. aeruginosa. Polymyxins also promote the release of histamine and serve as safe mucosal adjuvants that enhance vaccine-induced antigen-specific immune responses (for example ovalbumin intranasally immunized mice; Yoshino N et al., 2013). Updated 1/2014; 8/2015; 11/2015; 4/2017; 12/2017 GW.

 
       
 

Title:

Polymyxin: a new chemotherapeutic agent.

 
 

Author:

STANSLY PG, SHEPHERD RG, WHITE HJ.1947

 
 

Reference:

Bull Johns Hopkins Hosp. 1947 Jul;81(1):43-54. Pub-Med.

 
       

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