Antimicrobial Peptide AP01724“>017242788.23 %100.0 %AVP
     
 

APD ID:

AP01724

 
 

Name/Class:

Capistruin ( lactam; a class 2 lasso peptide; class 1 microcin, bacteriocins; Gram-negative bacteria, prokaryotes; XXJ; UCSB1a; BBpol)

 
 

Source:

Burkholderia thailandensis E264

 
 

Sequence:

GTPGFQTPDARVISRFGFN

 
 

Length:

19

 
 

Net charge:

1

 
 

Hydrophobic residue%:

31%

 
 

Boman Index:

1.93 kcal/mol

 
 

3D Structure:

Beta

 
 

Method:

X-ray

 
 

SwissProt ID:

PDB ID: 6N61   Go to PDB

 
 

Activity:

Anti-Gram-,

 
 

Crucial residues:

Residues Gly1, Arg11, Val12, and Ile13 were found to be critical for peptide maturation (Knappe TA et al 2009 Chem Biol 16: 1290-1298).

 
 

Additional info:

Discovered by genome mining based on the known MccJ25-gene coding cluster McjABCD. Capistruin is endoded by the capABCD gene cluster, where capA encodes the precursor polypeptide, capB cleaves the presursor and is likely to promote the cyclization, capC encodes an enzyme that activates the side chain of Asp9 for cyclization, and capD encodes a immunity protein. Showed activity against closely related Burkholderia and Pseudomonas strains. Different from the lasso structure of MccJ25, where the N-terminal Gly forms an amide bond with the carboxyl group of Glu8, capistruin forms a 9-residue macrolactam ring formed between NH of Gly1 and the carboxylic side chain of Asp9. In addition, Arg15 is reponsible for the trapping of the tail in the ring structure.

The structure of the peptide in complex with the RNA polymerase has been solved by X-ray crystallography (). Like MccJ25, Cap binds within the RNAP secondary channel. You can rotate, zoom, and view the 3D structure here in the PDB.Updated Jan2019.

 
       
 

Title:

Isolation and structural characterization of capistruin, a lasso peptide predicted from the genome sequence of Burkholderia thailandensis E264

 
 

Author:

Knappe TA, Linne U, Zirah S, Rebuffat S, Xie X, Marahiel MA.2008

 
 

Reference:

J Am Chem Soc. 2008 Aug 27;130(34):11446-54. PubMed

 
       

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