Antimicrobial Peptide AP01239

     
 

APD ID:

AP01239

 
 

Name/Class:

Cathelicidin-BF (BF-30; cathelicidin-WA; snake cathelicidin, reptiles, animals; Derivatives: BF-15; Cbf-K16)

 
 

Source:

venom Bungarus fasciatus

 
 

Sequence:

KFFRKLKKSVKKRAKEFFKKPRVIGVSIPF

 
 

Length:

30

 
 

Net charge:

11

 
 

Hydrophobic residue%:

40%

 
 

Boman Index:

2.12 kcal/mol

 
 

3D Structure:

Helix

 
 

Method:

NMR

 
 

SwissProt ID:

Reference ID: Ref  

 
 

Activity:

anti-Gram+ & Gram-, antifungal, Enzyme inhibitor, Cancer cells

 
 

Crucial residues:

 
 

Additional info:

The predicted mature sequence has 34-aa. The isolated peptide with N-terminal 4-aa truncation might be a further processed product. Since 2016, cathelicidin-BF is also called cathelicidin-WA (reason unknown). Importantly, it showed low toxicity against human blood cells. NMR structure in TFE was not presented due to incomplete restraints. Segments KF1-11, KF12-19, and KF20-30 had no activity. Active against E. coli ATCC25922 (MIC 2.3 uM), P. aeruginosa ATCC278953 (MIC 1.2 uM), S. aureus ATCC2592, and C. albicans (MIC 4.7 uM). Salt-insensitive: The activities did not change much in the presence of 150 mM NaCl. The peptide and its lysine 16 variant also possesses anticancer effects (Tian Y et al. 2013). A derivative called BF-15 was designed. BF-15 is composed of the N-terminal 10 amino acid residues (162KFFRKLKKSV172 of the precursor) of the peptide and another successive linked 5 amino acid residues (157VKRFK161 of the precursor) from the precursor amino acid sequence of BF-30. This peptide also inhibits E. coli ATP synthase (Azim et al., 2016). Cbf-K(16), Cbf-A(7), and Cbf-A(13) were obtained by mutating Lys(16), Ala(7), and Ala(13) of BF-30, respectively. Active against E. coli BL21 (DE3)-NDM-1 (Hao Q et al., 2013). The BF-30 K16 mutant also inhibits non-small cell lung cancer (Tian et al., 2013). Animal model:mouse. i.v. injection did not show CFU reductiomn. However, pretreatment with cathelicidin-BF ameliorates P. aeruginosa pneumonia in mice by enhancing NETosis and the autophagy of recruited neutrophils and macrophages (Liu C et al., 2018). Updated 3/2016; 6/2016; 11/2018.

 
       
 

Title:

Snake cathelicidin from Bungarus fasciatus is a potent peptide antibiotics.

 
 

Author:

Wang Y, Hong J, Liu X, Yang H, Liu R, Wu J, Wang A, Lin D, Lai R2008

 
 

Reference:

PLoS ONE. 2008 Sep 16;3(9):e3217. Pub-Med

 
       

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