Antimicrobial Peptide AP00895

     
 

APD ID:

AP00895

 
 

Name/Class:

OH-CATH (O. hannah cathelicidin, elapid cathelicidin, snake, reptiles, animals; Derivatives: OH-CATH30; OH-CM6)

 
 

Source:

king cobra, Ophiophagus hannah

 
 

Sequence:

KRFKKFFKKLKNSVKKRAKKFFKKPRVIGVSIPF

 
 

Length:

34

 
 

Net charge:

15

 
 

Hydrophobic residue%:

38%

 
 

Boman Index:

2.27 kcal/mol

 
 

3D Structure:

Helix

 
 

SwissProt ID:

Reference ID: Ref  

 
 

Activity:

anti-Gram+ & Gram-, Enzyme inhibitor,

 
 

Crucial residues:

 
 

Additional info:

Rich in lysines (35%). Active against E. cloacae (MIC 1 ug/ml), E. aerogenes (MIC 4 ug/ml), E. coli (MIC 6-20 ug/ml), S. aureus (MIC 4 ug/ml), and P. aeruginosa PAO1 (MIC 2 ug/ml). Predicted to be helical based on the known structure of human LL-37. OH-CATH30 is obtained by removing the N-terminal 4 residues. OH-CM6 is obtained by further shortening OH-CATH30 by deleting the C-terminal 10 residues followed by mutations. These derivatives are active against indicator and resistant bacterial strains (MIC in the range of 1.5-25 ug/ml). Activity is reduced in the presence of serum. However, the D-form is stable for at least 12 h. These peptides appear to work on membranes as indicated by rapid killing in 60 min (2XMIC) and dye leakage. Both OH-CATH30 and OH-CM6 displayed in vivo efficacy in reducing drug-resistant E. coli burdens in the neutropenic thigh infection animal model:mouse or murine bacteremia model. It also showed protective effects on E. coli induced rabbit urinary tract infection models (animal model:rabbit)( Zhang et al., 2013). The D-form of these peptides is also active in vivo although with higher cytotoxicity ( Li SA et al. 2010). In addition, topical application of OH-CATH30 is efficacious against drug-resistant P. aeruginosa keratitis ( Li SA et al., 2014). This peptide also inhibits Escherichia coli ATP synthase (Azim et al., 2016). Updated 13/4/2012; Feb2013; 3/2014; 3/2016 GW.

 
       
 

Title:

Identification and characterization of novel reptile cathelicidins from elapid snakes.

 
 

Author:

Zhao H, Gan TX, Liu XD, Jin Y, Lee WH, Shen JH, Zhang Y.2008

 
 

Reference:

Peptides. 2008 Oct;29(10):1685-1691. PubMed.

 
       

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