Antimicrobial Peptide AP00871

     
 

APD ID:

AP00871

 
 

Name/Class:

Temporin 1OLa (XXA, Temporin-1OLa, UCLL1c; frog, amphibians, animals. More AMPs?)

 
 

Source:

Florida bog frog, Rana okaloosae, North America

 
 

Sequence:

FLPFLKSILGKIL

 
 

Length:

13

 
 

Net charge:

3

 
 

Hydrophobic residue%:

61%

 
 

Boman Index:

-1.68 kcal/mol

 
 

3D Structure:

Helix

 
 

Method:

NMR

 
 

SwissProt ID:

Reference ID: Ref  

 
 

Activity:

Anti-Gram+, Antifungal, Anti-MRSA, Antibiofilm, Anticancer

 
 

Crucial residues:

 
 

Additional info:

C-terminal NH2. Antinacterial activity was not reported due to a limited amount of material. We filled up this gap by using a synthetic peptide. Active against S. aureus USA300 (MIC 1.6-3.1 uM), S. epidermidis 1457 (MIC 1.6uM), B. subtilis 168 (MIC 12.5-25 uM), C. albicans (MIC 12.5 uM), C. glabrata (MIC 12.5 uM), and C. tropicalis (1.6 uM). KIll HeLa cancer cells at 25 uM (Mishra, Wang et al. 2018). NMR analysis reveals a hydrophobic patch (residues 1-2) followed by a classic 2-turn amphipathic helix. Animal model:wax moth: Treatment with this peptide at a single dose at 32 mg/kg ahead of MRSA USA300 infection protected Galleria mellonella from infection-caused deaths. Updated 5/2018; 6/2019.

 
       
 

Title:

Peptidomic analysis of skin secretions from Rana heckscheri and Rana okaloosae provides insight into phylogenetic relationships among frogs of the Aquarana species group

 
 

Author:

Conlon JM, Coquet L, Leprince J, Jouenne T, Vaudry H, Kolodziejek J, Nowotny N, Bevier CR, Moler PE.2007

 
 

Reference:

Regul Pept 2007; 138: 87-93. PubMed.

 
       

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