Antimicrobial Peptide AP00548

     
 

APD ID:

AP00548

 
 

Name/Class:

Chicken CATH-2 (chicken cathelicidin 2; CMAP27, chicken myeloid antimicrobial peptide 27, Fowlicidin-2; chCATH-2; birds, animals; BBL; Derivatives: F2,5,12W)

 
 

Source:

Gallus gallus

 
 

Sequence:

RFGRFLRKIRRFRPKVTITIQGSARFG

 
 

Length:

27

 
 

Net charge:

9

 
 

Hydrophobic residue%:

37%

 
 

Boman Index:

3.3 kcal/mol

 
 

3D Structure:

Helix

 
 

Method:

NMR

 
 

SwissProt ID:

PDB ID: 2GDL   Go to PDB

 
 

Activity:

Anti-Gram+ & Gram-, Anti-MRSA, Hemolytic, Antibiofilm,

 
 

Crucial residues:

Proline and amidation

 
 

Additional info:

Active against E. coli O157:H7, S. typhimurium, K. pneumoniae, P. aeruginosa (MIC 0.6-5.2 uM), L. monocytogenes, S. aureus (MRSA) (MIC 0.30-1.3 uM). In a subsequent work, Xiao Y et al 2006 J Biol Chem 281: 2858-67) cloned a 32-residue peptide, which contains additional LVQRG at the N-terminus compared to CMAP27. Which one corresponds to the native CATH-2 is not yet clear. The additional five residues appeared not to have a significant effect on antimicrobial activity (Dijk A et al 2009 Mol Immun. 46: 2465-2473). For CAMP-27, a change of the last Gly to NH2 (amidation) greatly augmented antibacterial activity against E. coli, to a lesser degree against S. aureus, but not against B. globigii (van Dijk et al 2009 Mol Immunol 46: 2465-73). Antibacterial activities of other synthetic peptides: the N-terminal 15 residues are most potent, peptide residues 1-21 and peptide residues 12-26 are weakest; substitution of P by G only reduced activity slightly, but a change of Pro by Leu greatly reduced antibacterial activity. The potent peptides can kill bacteria rapidly in 5-10 minutes. Of interest, peptides corresponding to residues 1-15 or residues 12-26 showed poor hemolytic activity. Xiao et al (2008 J Innate Immu 1:268) also showed that the Pro residue is important for both antimicrobial activity and LPS neutralization.You can rotate, zoom, and view the 3D structure here in the PDB . Substitution of the three F2, F5, and F12 residues with W (peptide RWGRWLRKIRRWRPK) further improved antimicriobial and LPS-neutrolizing activities. This peptide derivative inhibits S. epidermidis biofilm formation at 2.5 uM (four times below MIC) based on CV staining. It impairs existing biofilms. Updated 2/2014; 6/2018.

 
       
 

Title:

CMAP27, a novel chicken cathelicidin-like antimicrobial protein

 
 

Author:

van Dijk A, Veldhuizen EJA, van Asten AJAM, Haagsman HP2005

 
 

Reference:

Vet. Immunol. Immunopathol. 106 (3-4), 321-327 (2005)

 
       

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