Antimicrobial Peptide AP00538

     
 

APD ID:

AP00538

 
 

Name/Class:

Halocidin (1S=S heterodimer Hal18 + Hal15; UCSS2a; tunicate, marine invertebrates, animals; Derivatives: HG1; Haloganan; Khal)

 
 

Source:

hemocytes, Halocynthia aurantium

 
 

Sequence:

WLNALLHHGLNCAKGVLA

 
 

Length:

18

 
 

Net charge:

1

 
 

Hydrophobic residue%:

61%

 
 

Boman Index:

-0.63 kcal/mol

 
 

3D Structure:

Unknown

 
 

SwissProt ID:

Reference ID: Ref  

 
 

Activity:

Anti-Gram+ & Gram-, Antifungal, Anti-MRSA,

 
 

Crucial residues:

 
 

Additional info:

Shown is the sequence of chain A. The sequence of chain B is ALLHHGLNCAKGVLA, which is an N-terminally 3-residue truncated version of the chain A. AMP stability: Shin YP et al. (2010 Antimicrob Agents Chemother 54:2855-66) showed that appending a Lys at the N-terminus of chain A and making chain B identical to the new chain A, leading to a peptide called HG1 that is resistant to proteases such as trypsin, chymotrypsin, and MMP-7. Animal model:mouse: a modified form of halocidin, with one additional lysine at the N-terminus of the long chain, is named as Khal, which is active against MRSA, VRE (anti-VRE) and L. monocytogenes in vitro. When treated intravenously, it showed ~1 log CFU reduction in liver and spleen when L. monocytogenes was infected intravenously via the tail. (Jang et al. 2007 ). An HG1 isomer with two substitutions of Gln-3 and Lys-19, named haloganan, showed an advantage over HG1 in that it exerted its potent antimicrobial activity under conditions containing human serum (serum-less susceptible) and/or wound fluid (Shin et al., 2014). Updated 10/8/2010; 12/2014 GW.

 
       
 

Title:

Halocidin: a new antimicrobial peptide from hemocytes of the solitary tunicate, Halocynthia aurantium

 
 

Author:

Jang WS, Kim KN, Lee YS, Nam MH, Lee IH2002

 
 

Reference:

FEBS Lett. 2002 Jun 19;521(1-3):81-6.

 
       

Close this window