Antimicrobial Peptide AP00513

     
 

APD ID:

AP00513

 
 

Name/Class:

Ranalexin (frog, amphibians, animals; ZZP; XXU; 1S=S, UCSS1a)

 
 

Source:

Bull frog, Rana catesbeiana, North America

 
 

Sequence:

FLGGLIKIVPAMICAVTKKC

 
 

Length:

20

 
 

Net charge:

3

 
 

Hydrophobic residue%:

65%

 
 

Boman Index:

-1.34 kcal/mol

 
 

3D Structure:

Helix

 
 

Method:

NMR

 
 

SwissProt ID:

SwissProt ID: P39084  Go to SwissProt

 
 

Activity:

Anti-Gram+ & Gram-, Antifungal, Antiparasitic, Anti-MRSA, Anticancer

 
 

Additional info:

Recombinant peptide is as active as the WT against S. aureus, S. pyogenes, E. coli, and MRSA (MIC 8 and 128 ug/ml). The recombinant ranalexin is also cytotoxic in HeLa and COS7 human cancer cells (IC50 =13-15 ug/ml)(Appl Microbiol Biotechnol. 2012). In membrane-mimetic TFE or DPC micelles, residues 8-15 are helical (edited 11/4/2007 GW). In an animal model:mouse for systemic infection, the peptide showed synergistic effect against MRSA with antistaphylococcal endopeptidase (Desbois AP et al., 2010 Int J Antimicrob Agents 35: 559-65). Updated 10/12/2012 GW.

 
       
 

Title:

Ranalexin, a novel antimicrobial peptide from bullfrog (Rana catesbeiana) skin, structurally related to the bacterial antibiotic, polymyxin

 
 

Author:

Clark DP, Durell S, Maloy WL, Zasloff M1994

 
 

Reference:

J Biol Chem 1994; 269: 10849-10855

 
       

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