Antimicrobial Peptide AP00473

     
 

APD ID:

AP00473

 
 

Name/Class:

Piscidin 1 (piscidin-1; Pis-1; fish, animals; Variants: Piscidin-1N, piscidin-1H; ZZH; BBII, UCLL1)

 
 

Source:

mainly mast cells, gill, skin, intestine, spleen, and anterior kidney, hybrid striped bass (Morone saxatilis x Morone chrysops); Morone saxatilis

 
 

Sequence:

FFHHIFRGIVHVGKTIHRLVTG

 
 

Length:

22

 
 

Net charge:

3

 
 

Hydrophobic residue%:

45%

 
 

Boman Index:

0.7 kcal/mol

 
 

3D Structure:

Helix

 
 

Method:

NMR

 
 

SwissProt ID:

PDB ID: 2jos   Go to PDB

 
 

Activity:

Anti-Gram+ & Gram-, Antiviral, Antifungal, Anti-HIV, Anti-MRSA, Hemolytic, Anticancer

 
 

Additional info:

First AMP isolated from mast cells. Active against E. coli, P. aeruginosa, S. typhimurium, B. subtilis, and S. aureus (MIC 1-4 uM) (Kim JP et al 2010), including MRSA USA300 (MIC 3.1 uM) (Menousek J et al 2012 ). It is also active against channel catfish virus (CCV), frog virus 3 (FV3) (Chinchar VG et al. 2004) as well as HIV-1 (EC50= 1.4 uM (Wang, G et al., 2010). The activity reported by Chinchar et al. did not depend on peptide C-terminal amidation (piscidin-1N: c-terminal amidation; piscidin-1H, no amidation. Note that both forms can occur in nature). For structure in SDS micelles, refer to Lee SA et al. 2007 Biochemistry 46: 3653-63. For structure in DPC, see Campagna S et al. 2007 Biochemistry 46, 1771-8. You can rotate, zoom, and view the 3D structure here in the PDB. Structure by solid-state NMR is here.Substitution of Gly8 with Pro8 or a Lys peptoid residue improved peptide selectivity. It was proposed that conformational flexibility and positive charge are important for membrane penetration (Kim JP et al 2010). This peptide also binds to metal Cu2+ (Rai et al., 2018). Updated 11/2012; 2/2014; 9/2014; Jan2019 GW.

 
       
 

Title:

Peptide antibiotics in mast cells of fish.

 
 

Author:

Silphaduang U, Noga E.J.2001

 
 

Reference:

Nature 2001; 414, 268 - 269; doi:10.1038/35104690. (PubMed)

 
       

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