Antimicrobial Peptide AP00447

     
 

APD ID:

AP00447

 
 

Name/Class:

Anoplin (insects, arthropods, invertebrates, animals; XXA; SAR; derivatives)

 
 

Source:

Solitary wasp Anoplius samariensis

 
 

Sequence:

GLLKRIKTLL

 
 

Length:

10

 
 

Net charge:

4

 
 

Hydrophobic residue%:

50%

 
 

Boman Index:

0.3 kcal/mol

 
 

3D Structure:

Helix

 
 

Method:

NMR

 
 

SwissProt ID:

PDB ID: 2MJQ   Go to PDB

 
 

Activity:

Anti-Gram+ & Gram-,

 
 

Crucial residues:

C-terminal NH2, hydrophobic Leu and Ile, and peptide length (Ifrah D et al 2005 J Pept Sci 11: 113-121)

 
 

Additional info:

It is helical in SDS or TFE based on CD. 3D structure of anoplin in complex with DPC micelle is determined by 2D NMR (NMR_DPC)(Uggerhøj LE et al., 2014). You can rotate, zoom, and view the 3D structure on a micelle here in the PDB. The peptide appears to present ion channel-like activity (Dos Santos Cabrera MP et al 2008 J Pept Sci 14: 661-9). However, C-terminal amidation is not important for antifungal activity (BBA 2014). In the above 2014 NMR study, the structure-activity relationship (SAR) was explored, including the determination of the 3D structures of several mutants (R5W; R5K, T8W; and R5F, T8W). Surface immobilization to chitosan polymers enahnced peptide activity and reduced peptide toxicity (Sahariah et al., 2015). Updated 2/2014; structure 12/2014; Jan2018.

 
       
 

Title:

Anoplin, a novel antimicrobial peptide from the venom of the solitary wasp Anoplius samariensis.

 
 

Author:

Konno K, Hisada M, Fontana R, Lorenzi CC, Naoki H, Itagaki Y, Miwa A, Kawai N, Nakata Y, Yasuhara T, Ruggiero Neto J, de Azevedo WF Jr, Palma MS, Nakajima T.2001

 
 

Reference:

Biochim Biophys Acta 2001;1550(1): 70-80

 
       

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