Antimicrobial Peptide AP00214

     
 

APD ID:

AP00214

 
 

Name/Class:

Tachyplesin I (Tac; TP1; UCSS1a; Horseshoe Crab, arachnids, Chelicerata, arthropods, invertebrates, animals, XXA, ZZHa; BBMm; BBL; 2S=S)

 
 

Source:

hemocytes, Southeast Asia, Tachypleus tridentatus; Tachypleus gigas; Carcinoscorpius rotundicauda

 
 

Sequence:

KWCFRVCYRGICYRRCR

 
 

Length:

17

 
 

Net charge:

7

 
 

Hydrophobic residue%:

47%

 
 

Boman Index:

3.53 kcal/mol

 
 

3D Structure:

Beta

 
 

Method:

NMR

 
 

SwissProt ID:

PDB ID: 1WO1   Go to PDB

 
 

Activity:

Anti-Gram+ & Gram-, Antiviral, Anti-HIV, Hemolytic, Anticancer

 
 

Crucial residues:

S-S bonds

 
 

Additional info:

Active against S. aureus209P (MIC3.1ug/ml), S. typhimurium LT2(S), S. minnesota 1114W(S) (MIC3.1-6.3ug/ml), S. typhimurium SL1102 (Re) (MIC0.8-1.6ug/ml), S. aureus ATCC 25923 (MIC6.3ug/ml), S. minnesota R595 (Re) (MIC3.1-12.5ug/ml) and E. coli K12 (MIC6.3-12.5ug/ml). Two disulfide bridges: 3,16; 7,12; beta hairpin conformation in DPC micelles (in water, PDB ID 1WO0). You can rotate, zoom, and view the 3D structure here in the PDB. Structure bound to LPS is here. Structure-activity relationship study improved peptide therapeutic index and reveals the importane of the beta-strands for stability (Edwards et al. 2017). MOA: it acts on bacterial membranes by forming a pore. Tac appears to influence the pathway underlying unsaturated fatty acid biosynthesis related to membrane function (Liu et al., 2018). It inhibits HSV-1, HSV-2 (Yasin et al., 2000), an enveloped DNA virus of Singapore grouper iridovirus (SGIV) and a non-enveloped RNA virus of viral nervous necrosis virus (RGNNV) (Xie H et al., 2016). MOA: Its anti-HIV activity may be due to the inhibition of virual adsorption to cells (Morimoto et al 1991 Chemotherapy 37:206-11). Transgenic plants: expression of this peptide in patato improves resistance to pathogens (Montesinos E 2007 FEMS Microbiol Lett 270, 1-11). The peptide has been expressed as a fusion protein and coated to haloarchaea-produced poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBHV) via hydrophobic interaction (Xue et al., 2018). Found in multiple species. Ref Updated 12/2012; 2/2014; 11/2015; 9/2016; 5/2018; 4/2019 GW/CW.

 
       
 

Title:

Tachyplesin, a class of antimicrobial peptide from the hemocytes of the horseshoe crab (Tachypleus tridentatus). Isolation and chemical structure

 
 

Author:

Nakamura T, Furunaka H, Miyata T, Tokunaga F, Muta T, Iwanaga S, Niwa M, Takao T, Shimonishi Y.1988

 
 

Reference:

J Biol Chem. 1988 Nov 15;263(32):16709-13.PubMed.

 
       

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