Antimicrobial Peptide AP00191

     
 

APD ID:

AP00191

 
 

Name/Class:

Gomesin (Gm, UCSS1a; Spiders, XXA, ZZP, XXQ, arachnids, Chelicerata, arthropods, invertebrates, animals; 2S=S; UCSS1a)

 
 

Source:

Hemocytes, Acanthoscurria gomesiana

 
 

Sequence:

QCRRLCYKQRCVTYCRGR

 
 

Length:

18

 
 

Net charge:

6

 
 

Hydrophobic residue%:

33%

 
 

Boman Index:

4.39 kcal/mol

 
 

3D Structure:

Beta

 
 

Method:

NMR

 
 

SwissProt ID:

PDB ID: 1KFP   Go to PDB

 
 

Activity:

Anti-Gram+ & Gram-, Antifungal, Antiparasitic, Antimalarial, Hemolytic, Anticancer

 
 

Crucial residues:

disulfide bonds (Fazio et al 2006 Biopolymers 84: 205-218)

 
 

Additional info:

Active against a vareity of bacteria: Gram+ A. viridans, B. cereus, B. megaterium, B. thuringiensis, E. faecalis, L. monocytogenes, M. luteus, P. acidolacrici, S. aureus, S. epidermidis, S. haemolyticus, s. saprophyticus, S. pyogenes, N. asteroides (MIC 0.4-12.5 uM); Gram- A. tumefaciens, E. coli 1106, E. coli D22, E. coli D31, E. coli SBS363, E. carolovora carolovora, E. cloacae b12, K. pneumoniae, P. aeruginosa, S. thyphinurium, X. campestris (MIC 0.4-6.2 uM); fungi A. brassicola, A. fumigatus, F. culmorum, F. oxysporum, N. crassa, N. haematococca, T. viridae, T. mentagrophytes; yeasts C. albicans, C. tropicalis, C. neoformans, S. cerevisiae (MIC 0.4-6.2 uM), C. glabrata, and B. bassiana (MIC 12.5-25 uM). Also active against P. falciparum. Gomesin bound to the cell surface of cryptococci neoformans, which resulted in cell death associated with membrane permeabilization (FEMS Microbiol Lett. 2007 Sep;274(2):279-86). Also, this peptide triggers SH-SY5Y cell death through L-type channel calcium influx, MAPK/ERK, PKC and PI3K signaling and generation of reactive oxygen species (Soletti RC et al 2010 Chem Biol Interact 186: 135-43). Although represented by E, the N-terminal residue is a pyroglutamate. The 3D structure was determined by 2D NMR (Eur J Biochem. 2002 Feb;269(4):1190-8). You can rotate, zoom, and view the 3D structure here in the PDB . Updated 5/2010; 2/2014; 5/2015. GW.

 
       
 

Title:

Isolation and characterization of gomesin, an 18-residue cysteine-rich defense peptide from the spider Acanthoscurria gomesiana hemocytes with sequence similarities to horseshoe crab antimicrobial peptides of the tachyplesin family.

 
 

Author:

Silva PI Jr, Daffre S, Bulet P.2000

 
 

Reference:

J Biol Chem. 2000 Oct 27;275(43):33464-70. PubMed.

 
       

Close this window