Antimicrobial Peptide AP00181

     
 

APD ID:

AP00181

 
 

Name/Class:

human defensin 6 (HD-6, HD6, intestinal, alpha defensin; UCSS1a; primates, mammals, animals; BBBH2O; 3S=S)

 
 

Source:

Paneth cells, intestine, Homo sapiens

 
 

Sequence:

AFTCHCRRSCYSTEYSYGTCTVMGINHRFCCL

 
 

Length:

32

 
 

Net charge:

2

 
 

Hydrophobic residue%:

40%

 
 

Boman Index:

1.71 kcal/mol

 
 

3D Structure:

Beta

 
 

Method:

X-Ray

 
 

SwissProt ID:

PDB ID: 1ZMQ   Go to PDB

 
 

Activity:

Antiviral, Antifungal,

 
 

Crucial residues:

His27

 
 

Additional info:

Constitutively expressed. A reduced expression is observed in ileal Crohn's disease (Wehkamp J et al., 2005). Three disulfide bridges. Three disulfide bridges. It had been puzzling regarding the role of this defensin. Recent studies revealed two mechanisms. First, HD6 is now shown to control enteric bacterial pathogens in vitro and in vivo by binding to bacterial surface proteins. The peptide appears to be able to form fibrils and nanonets that surround and entangle bacteria. This self-assembly mechanism occurs in vivo, requires histidine-27, according to x-ray crystallographic studies. You can rotate, zoom, and view the crystal structure of the wild-type HD-6 here in the PDB. You can also rotate, zoom, and view the 3D structure of the His27 mutant here. However, you need look at the figures in the published paper for the crystal packing of the monomers. These findings support a key role for HD6 in protecting the small intestine against invasion by diverse enteric pathogens and may explain the conservation of HD6 throughout Hominidae evolution Science 2012. Second, HD-6 is a true AMP that kills bacteria under a reduced environmental conditions (Schroeder BO et al., 2014). Activity was observed against anaerobic gut commensals but not against some pathogenic strains. An APD recommended reading. APD update 6/2012; 2/2014; 2/2015; 10/2015. GW.

 
       
 

Title:

Defensin-6 mRNA in human Paneth cells: implications for antimicrobial peptides in host defense of the human bowel.

 
 

Author:

Jones DE, Bevins CL.1993

 
 

Reference:

FEBS Lett. 1993; 315:187-192

 
       

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