Antimicrobial Peptide AP00166

     
 

APD ID:

AP00166

 
 

Name/Class:

Pleurocidin (NRC-4, NRC-04; XXA; fish, animals)

 
 

Source:

the skin mucous secretions, Winter flounder, Pleuronectes americanus

 
 

Sequence:

GWGSFFKKAAHVGKHVGKAALTHYL

 
 

Length:

25

 
 

Net charge:

4

 
 

Hydrophobic residue%:

44%

 
 

Boman Index:

0.2 kcal/mol

 
 

3D Structure:

Helix

 
 

Method:

NMR

 
 

SwissProt ID:

PDB ID: 1Z64   Go to PDB

 
 

Activity:

anti-Gram+ & Gram-, Chemotactic, Anti-MRSA, Antibiofilm,

 
 

Additional info:

Active against E. coli (MIC 2.2-3.3 uM), A. salmonicida (MIC 17.7-35 uM), C. aquatilis (MIC 2.2-4.4 uM), P. haemolytica (MIC 4.4-8.8 uM), S. typhimurium I or II (MIC 8.8-17.7 uM), Gram-positive B. subtilis (MIC 1.1-2.2 uM), S. aureus (MIC 17.7-35 uM). In addition, it is active against oral bacteria S. mutans (UA 159, UA 140) (MIC 8-16 ug/ml), S. sanguinis ATCC 10556; S. sobrinus ATCC 6715, S. gordonii ATCC 10558, L. casei ATCC 393, L. fermenti (MIC 2-32 ug/ml) (Tao R et al., 2011). Also, this peptide kills the biofilms of S. mutans UA159 (~50% killed at 32 ug/ml based on SYT09 and PI staining). Structure in TFE or DPC was reported by Syvitski RT et al 2005 Biochemistry 44: 7282-93. You can rotate, zoom, and view the 3D structure here in the PDB . Truncation of either end (i.e. decrease in hydrophobicity) reduces the activity of the peptide (Cho J et al., 2012). The sequence of NRC-4 (Patrzykat et al. 2003 ANTIMICROB AGENTS CHEMOTHER, 47: 24642470) is identical to this peptide. However, NRC-4 is C-terminally amidated and is active against A. salmonicida 99-1, A. salmonicida 97-4, S. enterica serovar Typhimurium MS7953s (MIC 2 uM), S. enterica serovar Typhimurium 14028s (MIC 16 uM), P. aeruginosa K799 (MIC 8 uM), P. aeruginosa Z61 (MIC 4 uM), E. coli CGSC4908 (MIC 2 uM), E. coli UB1005 (MIC 4 uM), E. coli DC2 (MIC 2 uM), S. epidermidis C621, MRSA C623, C. albicans C627 (MIC 8 uM). This peptide is chemotactic as well (Pundir P et al 2013). For other NRC peptides, see AP777-792. Updated 6/2013; 7/2013; 5/2015.

 
       
 

Title:

Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretion of winter flounder.

 
 

Author:

Cole AM, Weis P, Diamond G.1997

 
 

Reference:

J. Biol. Chem. 1997; 272:12008-12013. Pub-Med.

 
       

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