Antimicrobial Peptide AP00155

     
 

APD ID:

AP00155

 
 

Name/Class:

SMAP-29 (SMAP29, sheep myeloid AMP-29; SMAP-28, OaMAP28, ovine cathelicidin, sheep, ruminant, mammals, animals; BBomp; BBL; derivatives: Ovispirin, OV-1, OV-2, OV-3, novispirin, novici

 
 

Source:

sheep leukocytes; Ovis aries

 
 

Sequence:

RGLRRLGRKIAHGVKKYGPTVLRIIRIAG

 
 

Length:

29

 
 

Net charge:

9

 
 

Hydrophobic residue%:

37%

 
 

Boman Index:

2.16 kcal/mol

 
 

3D Structure:

Helix

 
 

Method:

NMR

 
 

SwissProt ID:

PDB ID: 1FRY   Go to PDB

 
 

Activity:

Anti-Gram+ & Gram-, Antifungal, Anti-MRSA, Hemolytic, Antibiofilm,

 
 

Additional info:

The peptide was also discovered by Mahoney M et al. in the same year.

Activity: Active against E. coli ATCC 25922, E. coli ML-35, E. coli D21, S. typhimurium ATCC 14028, P. aeruginosa ATCC 27853 (MIC 0.12-0.5 uM), P. aeruginosa (isolate from FC patient) (MIC 0.25-2 uM), S. marcescens ATCC 8100 (MIC 0.25 uM), S. aureus ATCC 25923, S. aureus Cowan 1, S. aureus (MRSA, clinical isolate) (MIC 0.5-1.0 uM), S. epidermidis ATCC 12228, E. faecalis ATCC 29212, E. faecalis (VREF, clinical isolate) (MIC 1 uM), B. megaterium Bm11, C. albicans (clinical isolate), C. neoformans (clinical isolate), and R. rubra. In the synthetic peptide used for activity assays, the last Gly residue was converted to NH2 (more precisely, SMAP-28). Removal of the C-terminal residues 18-28 or changing residues 22,25,and 26 to K disrupted hemolytic activity of the peptide (Shin et al 2001 BBRC 285: 1046). In TFE, residues 8-17 are helical, residues 18-19 serves as a hinge, and residues 20-28 are ordered (Tack BF et al. 2002 Eur J Biochem 269: 1181).

(MOA) Recently, it is proposed that SMAP-29 and hRNase 7 bind to an outer membrane protein I (OprI) of Gram-negative bacterium P. aeruginosa (Lin YM et al., 2010 J Biol Chem 285:8985). For another peptide working in a similar way, see the APD entry 431.

Structure: You can rotate, zoom, and view the 3D structure here in the PDB.

Immobilization: The peptide has been immobilized to superparamagnetic beads and glass with antimicorbial activity (Soares et al., 2015). Ovispirin-1 (KNLRRIIRKIIHIIKKYG) was designed based on SMAP-29.

Design: Ovispirin-1 (OV-1) is a broad-spectrum antimicrobial peptide similar to SMAP-29. OV-2 and OV-3 are shortened or mutated versions of OV-1 (Saiman L et al 2001 Antimicrob Agent Chemother. 45: 2838-44). However, novispirin, obtained by changing residue I10 of OV-1 to G10, can kill bacteria rapidly but is relatively nontoxic to human cells (Sawai MV et al 2002 Protein Engineering 15: 225-232). Novicidin (seq: KNLRRIIRKGIHIIKKYF) is another derivative of OV-1. Updated 8/2010; 6/2018.

 
       
 

Title:

cDNA sequences of three sheep myeloid cathelicidins

 
 

Author:

Bagella L, Scocchi M, Zanetti M.1995

 
 

Reference:

FEBS Lett. 1995 Dec 4;376(3):225-8. PubMed.

 
       

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