Antimicrobial Peptide AP00094

     
 

APD ID:

AP00094

 
 

Name/Class:

Temporin A (temporin-1Ta; temporin-Ta; TA; Leu-rich; XXA; UCLL1c; frog, amphibians, animals; ZZP)

 
 

Source:

European common frog, Rana temporaria

 
 

Sequence:

FLPLIGRVLSGIL

 
 

Length:

13

 
 

Net charge:

2

 
 

Hydrophobic residue%:

61%

 
 

Boman Index:

-1.54 kcal/mol

 
 

3D Structure:

Helix

 
 

Method:

NMR

 
 

SwissProt ID:

PDB ID: 2MAA   Go to PDB

 
 

Activity:

anti-Gram+ & Gram-, antiviral, antiparasitic, Chemotactic, wound healing,

 
 

Crucial residues:

Hydrophobic residues at positions 1,5, and 12 and a positive charge at 7 are critical for antibacterial activity.

 
 

Additional info:

C-terminal amidated. Active against B. megaterium Bm11, S. aureus Cowan1, Y. pseudotubercolosis, S. pyogenes beta hem. group A (MIC 1-2.3 uM), E. coli D21 (MIC 11.9 uM), and C. albicans (MIC 3.4 uM); also S. aureus (MIC ~3-8 uM), E. coli (MIC 6.2-100 uM), and A. baumannii (MIC 24 uM), including parasites L. donovani and L. mexicana (Molecules. 2015 Feb 6;20(2):2775-85; J Biol Chem. 2005 Jan 14;280(2):984-90). It is also active against channel catfish virus (CCV) as well as the non-enveloped frog virus 3 (FV3) (Chinchar VG et al. Virol. 323:268-275). For NMR studies in SDS and DPC micelles, see Carotenuto et al. 2008 (J Med Chem 51: 2354-62). You can rotate, zoom, and view the 3D structure bound to LPS here in the PDB. Transgenic plants: The small, naturally occurring antimicrobial cationic peptide, temporin A, was N-terminally modified (MsrA3) and expressed in potato plants. MsrA3 conveyed strong resistance to late blight and pink rot phytopathogens in addition to the bacterial pathogen Erwinia carotovora. Transgenic tubers remained disease-free during storage for more than 2 years (Transgenic Res. 2004 Apr;13(2):181-90). It also showed wound healing effect (Simonetti O et al., 2008). Updated 3/2015; Jan2017; 11/2017.

 
       
 

Title:

Temporins, antimicrobial peptides from the European red frog Rana temporaria.

 
 

Author:

Simmaco M, Mignogna G, Canofeni S, Miele R, Mangoni ML, Barra D.1996

 
 

Reference:

Eur J Biochem. 1996 Dec 15;242(3):788-92. PubMed.

 
       

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