Antimicrobial Peptide AP00080

     
 

APD ID:

AP00080

 
 

Name/Class:

Esculentin-1 (frog, amphibians, animals; ZZI; XXU; 1S=S, UCSS1a)

 
 

Source:

Edible frog, Rana esculenta, Europe

 
 

Sequence:

GIFSKLGRKKIKNLLISGLKNVGKEVGMDVVRTGIDIAGCKIKGEC

 
 

Length:

46

 
 

Net charge:

6

 
 

Hydrophobic residue%:

41%

 
 

Boman Index:

0.98 kcal/mol

 
 

3D Structure:

Helix

 
 

Method:

NMR

 
 

SwissProt ID:

SwissProt ID: P32414  Go to SwissProt

 
 

Activity:

Anti-Gram+ & Gram-, Antifungal, candidacidal, Insecticidal, anti-diabetes, Hemolytic,

 
 

Additional info:

Active against E. coli D 21(LC 0.2uM), B. megaterium BmII (LC 0.1uM), S. aureus Cowan 1(LC 0.4uM), P. aeruginosa ATCC15692 (LC 0.7uM), C. albicans (LC 0.5uM) and S. cerevisiae (LC 0.9uM). (MIC data provided by Chunfeng Wang). Residue 1-18 appears to contain the antimicrobial region that forms predominantly a helical conformation in TFE (Biopolymers2012). An amphibian antimicrobial peptide variant expressed in Nicotiana tabacum confers resistance to bacterial or fungal phytopathogens. Also showed insecticidal effect.Transgenic plants: A M28L mutant was expressed in plants. The morphology of transgenic plants was unaffected. Challenging these plants with bacterial or fungal phytopathogens demonstrated enhanced resistance up to the second generation. Moreover, transgenic plants (e.g. Nicotiana tabacum) displayed insecticidal properties (Biochem J. 2003 Feb 15;370(Pt 1):121-7). This peptide promotes insulin secretion in acute 20-min incubations with glucose responsive BRIN-BD11 cells (Marenah L et al., 2006), thereby of interest for type 2 diabetes (T2D). APD update 8/2012; 9/2017, 12/2018; 5/2020

 
       
 

Title:

Novel antimicrobial peptides from skin secretion of the European frog Rana esculenta.

 
 

Author:

Simmaco,M., Mignogna,G., Barra,D., Bossa,F.1993

 
 

Reference:

FEBS Lett. 1993; 324:159-161. PubMed.

 
       

Close this window