Antimicrobial Peptide AP00061

     
 

APD ID:

AP00061

 
 

Name/Class:

Maximin 4 (UCLL1c; XXA; Maximin-4; toad, amphibians, animals)

 
 

Source:

Chinese red belly toad, Bombina maxima , Yunnan, China, Asia

 
 

Sequence:

GIGGVLLSAGKAALKGLAKVLAEKYAN

 
 

Length:

27

 
 

Net charge:

4

 
 

Hydrophobic residue%:

51%

 
 

Boman Index:

-0.51 kcal/mol

 
 

3D Structure:

Helix

 
 

Method:

NMR

 
 

SwissProt ID:

PDB ID: 2MHW   Go to PDB

 
 

Activity:

anti-Gram+ & Gram-, antiviral, antifungal, anti-HIV, Cancer cells

 
 

Additional info:

Active against E. coli ATCC25922, S. aureus ATCC2592, B. pyocyaneus CMCCB10104, B. megatherium, B. dysenterium, K. pneumoniae, and C. albicans ATCC2002 (MIC 1.5-15 uM). active against cancer cells C8166 and Molt-4 (IC50 24-35 ug/ml). It also has anti-HIV and spermicidal effects. EC50 against HIV-1 is 21.9 ug/ml (cell selectivity index 1.1) (Lai R et al., 2012). A helix-break-helix structure was found in SDS micelles or methanol. You can rotate, zoom, and view the 3D structure in SDS micelleshere in the PDB . The N-terminus is highly disordered. Despite the similarities in the overall topology of the two structures, major differences were observed in terms of the interactions stabilizing the kink region and the arrangement of the four lysine residues. This has a marked influence on the shape and charge distribution of the molecule and may have implications for the bacterial selectivity of the peptide (Toke O et al. 2011 Eur Biophys J) . Updated 1/2011; 2/2012; 12/2015 GW.

 
       
 

Title:

Antimicrobial peptides from skin secretions of Chinese red belly toad Bombina maxima.

 
 

Author:

Lai R, Zheng Y-T, Shen J-H, Liu GJ, Liu H, Lee W-H, Tang S-Z., Zhang Y.2002.

 
 

Reference:

Peptides 2002; 23:427-435. PubMed.

 
       

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