Antimicrobial Peptide AP00035

     
 

APD ID:

AP00035

 
 

Name/Class:

Plantaricin A (PlnA, bacteriocin/pheromone, Gram-positive bacteria, prokaryotes; Other truncated forms: PlnA 23-mer; PlnA 22-mer)

 
 

Source:

Lactobacillus plantarum C11, WCFS1, V90

 
 

Sequence:

KSSAYSLQMGATAIKQVKKLFKKWGW

 
 

Length:

26

 
 

Net charge:

6

 
 

Hydrophobic residue%:

42%

 
 

Boman Index:

0.81 kcal/mol

 
 

3D Structure:

Helix

 
 

Method:

NMR

 
 

SwissProt ID:

PDB ID: 1YTR   Go to PDB

 
 

Activity:

anti-Gram+ & Gram-, Cancer cells

 
 

Additional info:

Antibacterial activity requires both chain a (PlnA) and chain b (PlnB). The sequence of chain a and chain b, however, are essential identical with the only difference being one additional Ala at the N-terminus of chain b. Active against P. pentosaceus Pac 1.0 (MIC 100 nM), L. plantarum 965 (MIC 300 nM), L. casei subsp. casei NCDO 161 (MIC 250 nM), L. sake NCDO 2714 (MIC 60 nM), and L. viridescens NCDO 1655 (MIC 20 nM). The peptide is disordered in water but became helical in membranes. The 3D structure of chain a in DPC micelles has been determined by NMR spectroscopy (J Biol Chem. 2005 Jun 17;280(24):22945-50). You can rotate, zoom, and view the 3D structure here in the PDB . The helical region (residues 12-21) are good for membrane targeting as well as the pheromone/hormone function. Ref for anticancer effect is here: S.L. Sand, T.M. Haug, J. Nissen-Meyer, O. Sand, The bacterial peptide pheromone plantaricin A permeabilizes cancerous, but not normal, rat pituitary cells and differentiates between the outer and inner membrane leaflet, J. Membr. Biol. 216 (2007) 6171. Found in multiple species. The N-terminal KSS segment is missing in PlnA 23mer, while the N-terminal KSSA is absent in PlnA 22mer. The 22mer showed a similar activity spectrum with reduced activity in certain cases. Updated 8/2010; 3/2013; 2/2014; 3/2016 GW.

 
       
 

Title:

Purification and characterization of plantaricin A, a Lactobacillus plantarum bacteriocin whose activity depends on the action of two peptides.

 
 

Author:

Nissen-Meyer J, Larsen AG, Sletten K, Daeschel M, Nes IF.1993

 
 

Reference:

J Gen Microbiol. 1993 Sep;139(9):1973-8. PubMed.

 
       

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