Antimicrobial Peptide AP00026

     
 

APD ID:

AP00026

 
 

Name/Class:

Lactoferricin B (LfcinB, UCSS1a; 1S=S, cattle, ruminant, animals, ZZHa; BBL; Derivatives: lactoferrin peptide 2; LTX-302; LfcinB6; MPLfcinB6; JJsn)

 
 

Source:

Bos taurus

 
 

Sequence:

FKCRRWQWRMKKLGAPSITCVRRAF

 
 

Length:

25

 
 

Net charge:

8

 
 

Hydrophobic residue%:

48%

 
 

Boman Index:

2.75 kcal/mol

 
 

3D Structure:

beta

 
 

Method:

NMR

 
 

SwissProt ID:

PDB ID: 1LFC   Go to PDB

 
 

Activity:

anti-Gram+ & Gram-, antiviral, antifungal, anti-HIV, Cancer cells

 
 

Crucial residues:

W6, W8

 
 

Additional info:

LfcinB is a 25-residue antimicrobial peptide released by pepsin cleavage of lactoferrin. Disulfide bond is not required for the antimicrobial potency. Active against E. coli, S. enteritidis, K. pneumoniae, P. vulgaris, Y. enterocolitica, P. aeruginosa, C. jejuni, S. aureus, S. mutans, C. diphtheriae, L. monocytogenes and Clostridium perfringens. Also active against E. coli ATCC 2592, M. luteus, and S. cerevisae (Ke T et al., 2012). Active against fungal biofilms (A. fumigatus, F. solani, and C. albicans) (Sengupta J et al., 2012). NMR structure: Residues 7-9 form beta1 and residues 17-19 form beta2 (in membrane). You can rotate, zoom, and view the 3D structure here in the PDB . This contrasts with X-ray structure where residues 1-13 is helical. Thus, this peptide appears to be able to adopt a different conformation depending on environmental conditions. lactoferrin (Lf) peptide 2 is a derivative corresponding to residues 1-10 of LfcinB (Ueta E, Tanida T, Osaki T.2001 A novel bovine lactoferrin peptide, FKCRRWQWRM, suppresses Candida cell growth and activates neutrophils. J Pept Res 2001 Mar;57(3):240-9). LfcinB6 (sequence: RRWQWR) corresponds to residues 4-9 of the peptide. Structure of LfcinB6 was determined in SDS micelles (Vogel HJ et al 2002 Biochem Cell Biol 80:49-63). Free LfcinB6 did not kill T-leukemia or breast cancer cells, but LfcinB6 was strongly cytotoxic when delivered to the cytosolic compartment by fusogenic liposomes (Richardson A 2009 Biochem Biophys Res Commun. 2009 Aug 21). In vivo efficacy:Animal model:mouse: Injection of a 9-mer derivative, LTX-302, led to tumor necrosis, infiltration of inflammation, and complete regression in the majority of mice (Berge G et al., 2010 Cancer Immunol Immunother 59: 1285-94). Appending hepta-arginine sequence via a glycine-glycine linker generates a more selective peptide MPLfcinB6 against human T-leukemia and B-lymphoma cells by damaging cell membranes (Hilchie AL et al., 2013). Lactoferrin shows synergistic effects with lysostaphin or nisin. Updated 3/2015; 6/2017; 11/2018.

 
       
 

Title:

Antibacterial spectrum of lactoferricin B, a potent bactericidal peptide derived from the N-terminal region of bovine lactoferrin.

 
 

Author:

Bellamy W, Takase M, Wakabayashi H, Kawase K, Tomita M.1992

 
 

Reference:

J Appl Bacteriol. 1992 Dec;73(6):472-9. PubMed.

 
       

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