Antimicrobial Peptide AP00010

     
 

APD ID:

AP00010

 
 

Name/Class:

BACTENECIN 7 (bac-7, bac 7; bac7; Pro-rich; bovine cathelicidin, ruminant, mammals; animals; BBL, SeqAR, BBPP; Derivatives: Bac7(1-35); Bac7(1-16), BBribo)

 
 

Source:

Bovine neutrophils, Bos taurus

 
 

Sequence:

RRIRPRPPRLPRPRPRPLPFPRPGPRPIPRPLPFPRPGPRPIPRPLPFPRPGPRPIPRPL

 
 

Length:

60

 
 

Net charge:

17

 
 

Hydrophobic residue%:

20%

 
 

Boman Index:

3.29 kcal/mol

 
 

3D Structure:

Rich

 
 

Method:

X-ray

 
 

SwissProt ID:

PDB ID: 5HAU   Go to PDB

 
 

Activity:

anti-Gram-,

 
 

Crucial residues:

N-terminal residues RRIR

 
 

Additional info:

Active against E. coli ATCC 25922, S. typhimurium LT2 (MIC 12 ug/ml), S. typhimurium ATCC 14028(MIC 25 ug/ml), K. pneumoniae ATCC 13883(MIC 12 ug/ml), E. cloacae ATCC 13047 (MIC 25 ug/ml) and P. aeruginosa ATCC 7700(MIC 50 ug/ml). (Mueller-Hinton broth)(provided by Chunfeng Wang). Rich in P and R. Bac7(1-35) has antibacterial activity similar to bac7. Also, Bac7(116) was an equally potent inhibitor as Bac7(135), consistent with the similar MICs observed for these two derivatives. Mechanism of action: At ~MIC, the fragment binds to internal targets via stereospecific uptake (because all-D-enantiomer failed to be taken in and much less active); at higher concentrations, it works via non-specific membranolytic mechanism. Bac(1-16) is still active against E. coli and S. enterica but kinetically deficient compared to bac(1-35). Bac(5-35) is much less active, indicating the importance of the N-terminal segment (Biochim Biophys Acta 2006; 1760: 1732-1740). Crystal structures of Bac 7 fragments in complex with E. coli Dnak have been solved [PDB ID 4JWC (use the PDB link below) for Bac7(1-16) and 4JWD for Bac7(15-28 )]. However, Bac7(1-35) is likely to bind to the 70S ribosome and inhibit protein synthesis Mario Mardirossian et al, 2014online. You can rotate, zoom, and view the structure of Bac7(1-35) in complex with the T. thermophilus 70S ribosome here in the PDB . By removing the first 4 critical residues, peptide Bac7(5-35) can only stabilize the initiation complex at a high concentration of 100 uM, providing a structural basis to the significance of the N-terminal segment (Seefeldt AC et al., 2016). Updated 10/15/2008; 12/2011; 12/2013; 2/2014; 11/2014; Jan2016; 6/2016.

 
       
 

Title:

Purification, composition, and activity of two bactenecins, antibacterial peptides of bovine neutrophils

 
 

Author:

Gennaro R, Skerlavaj B, Romeo D.1989

 
 

Reference:

Infect Immun. 1989 Oct;57(10):3142-6. PubMed.

 
       

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